1月7日电分析化学系列学术报告
报告题目：关于DNA荧光银纳米簇和DNA智能水凝胶的若干研究
报 告 人：郭玮炜博士
单 位：以色列耶路撒冷希伯来大学
报告时间：2015年1月7日（星期三）上午9:30
报告地点：长春应化所 教育大厦6040
报告人简介：郭玮炜博士，男，1980年4月出生，吉林大学化学专业本科，中科院长春应化所分析化学专业博士，导师汪尔康研究员，现在以色列耶路撒冷希伯来大学从事化学博士后研究工作。长期致力于DNA纳米技术，荧光贵金属纳米簇，DNA智能水凝胶等方面的研究，取得了多项创新成果。在Angew. Chem. Int. Ed., J. Am. Chem. Soc., Adv. Mater.等国际权威期刊发表学术论文22篇，被引用次数达760多次，h-index: 14。其中以第一作者发表文章10篇，总影响因子超过80，引用次数达370次。
报告摘要： DNA has also been developed as a powerful structural material for the functional construction from nanometer scale to bulk size since 1980s. My recent research activities are related to two aspects of the DNA based functional materials: the DNA templated fluorescent silver nanoclusters (Ag NCs) and the DNA based smart hydrogels. The Ag-NCs were applied as optical labels for sensing, biosensing, imaging and computing. In the past several years, we worked in this field and got a series of important progresses: (I) We observed that the fluorescence emission of DNA stabilized Ag NCs can be selectively quenched by Hg2+ pollutants. (II) We expanded the stabilizing templates of fluorescent DNA Ag NCs from single-stranded DNA strands to hybridized DNA duplexes. Furthermore, we developed a general method to indentify single nucleotide mutations based on the highly sequence dependent formation of fluorescent Ag NCs in these hybridized DNA duplex templates. (III) We developed a novel fluorescent Ag NCs based DNA molecular switch in which strand exchange reaction was applied to switch the fluorescent signal of DNA Ag NCs between “ON” and “OFF” states. (IV) The DNA stabilized Ag NCs were applied in the construction of one-dimensional DNA structures, the fluorescent DNA nanowires, and three-dimensional DNA structures, the fluorescent DNA hydrogels. (II) For the first time, poly-N-isopropylacrylamide (pNIPAM) polymer chains tethered with functional DNA strands were synthesized and applied to form hydrogels. The hydrogels exhibited switchable hydrogel-to-solution transitions and thermosensitive hydrogel-to-solid transitions. (III) We introduced a new concept to design stimuli-responsive switchable DNA-based hydrogels that exhibited shape-memory properties.
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